Scientists at leading research institutes and laboratories worldwide trust and depend on the superior results provided by Bioline reagents.

Achieve better results, faster and allow your resources to be used more efficiently with Bioline as your partner for research.

As a biologist you routinely face many research challenges, from low quality or limiting quantities of starting materials to low expression levels and instability of your samples. The pressure is inevitably on your time, valuable resources and budgets.

Across a wide range of applications and fields of research, from human and animal disease understanding to plant or virus pathways, the robust performance and tolerance of inhibitors demonstrated by Bioline reagents allows you to enhance experiments and generate meaningful data, faster and better than before.

So, if you want to improve your workflow, discover more, and save time and resource, choose Bioline as your skilled life science research partner for:

“The speed of MyTaq is surprisingly fast, only 20 seconds for 1.8kb with great amplifying efficiency!”

– Plant Research Centre, Adelaide, Australia

Plant material contains an abundance of potent PCR inhibitors such as polyphenols, tannins and complex polysaccharides, which make amplification of plant-derived DNA a challenging task.

MyTaq HS and ISOLATE II Plant DNA are new generations of high-performance reagents from Bioline, developed to deliver outstanding results on all templates including the complex genomic DNA templates found in plants. MyTaq is based on the latest technology in PCR enzyme preparation and engineered to increase affinity for DNA, which results in significant improvements to yield, sensitivity and speed.

ISOLATE II Plant DNA Kit is designed for the rapid purification of genomic DNA from a variety of wet or dry plant material, including leaves, bark, roots, fruits, etc. In order to show the suitability of MyTaq HS for the PCR of plant DNA, samples were initially prepared from a number of different plant types with the Bioline ISOLATE II Plant DNA Kit.

Key Solutions for Plant Research

Plant Expression Research Workflow

Plant Expression Research Workflow

Plant Genomic Research Workflow

Plant Genomic Research Workflow

As a researcher at the leading edge of virology research, your discoveries are transforming the diagnosis, treatment and prevention of viral disease. Whether studying viral genomics, molecular evolution and phylogenetics, interactions of virus and host genotypes, or the epidemiology of an emerging viral pathogen - which all require rapid and highly sensitive analysis of viral nucleic acids – Bioline is your trusted partner in virology discovery.

Bioline high-performance products are used in leading Viral Research and Surveillance Centres including:

  • WHO Collaborating Centre for Reference and Research on Influenza, Melbourne, Australia
  • Chinese Center for Disease Control and Prevention, Beijing, China
  • U.S. Army Medical Research Institute of Infectious Diseases (USAMRIID), Maryland, USA
  • Institute for Virology, Marburg, Germany

Key Solutions for Virology Research

Virology Workflow

Virology Workflow

The epigenome has been described as a new challenge in the post-genomic era. Modifications to the epigenome affecting gene expression include chromatin remodelling, noncoding RNA and DNA methylation.

Methylation of DNA is one of the best-studied epigenetic phenomena - bisulfite-modification of DNA being considered the ‘gold-standard’. However, bisulfite-modified DNA is difficult to amplify with other commercially available DNA polymerases, due to high levels of template degradation, in typically harsh DNA conversion conditions. In addition, the presence of uracil bases in modified DNA can cause enzyme stalling resulting in poor reaction success.

EPIK™ is a new generation of outstanding reliability and high performance molecular biology tools, engineered for epigenetic analysis.

Whether studying epigenetic processes essential for normal cellular development and differentiation, or epigenetic dysregulation important in the development of cancer, neurodegenerative diseases, metabolic disorders and inflammatory diseases, let Bioline be your partner in driving epigenetic discovery.

Key Solutions for Epigenetics Research

Epigenetics Workflow

Epigenetics Workflow

From identification of cancer-related genes to the discovery of new personalized, targeted therapies, your mission is to make the discoveries that defeat cancer. From integrative studies of genetic, epigenetic and gene expression signatures in cancer, your team is driving a ‘bench to bedside’ approach - rapidly pushing forward basic laboratory discoveries into real patient benefit.

Bioline offers exceptional, best-in-class tools to drive understanding of fundamental cancer cell biology, accelerate clinical and translational studies and catalyze novel biomarker discovery. Whether you require industry-leading solutions for performing rapid and high-yield nucleic acid isolation, highly sensitive molecular genetic/epigenetic profiling of tumors and cell lines, cloning of cancer genes, or tried and trusted kits for preclinical and biobank genotyping, Bioline is your proven partner for cancer research.

Key Solutions for Cancer Research

Cancer Workflow

Cancer Workflow

“SensiFAST consistently exhibits excellent sensitivity in our differential gene expression assays and superior efficiency compared with our previous qPCR mix.”

– National Institutes of Health
Bethesda, Maryland, USA

When your research is centered on gene expression analysis, Bioline is the perfect partner.

Start with ISOLATE II Kits for purification and concentration of high-integrity Total RNA from a wide range of starting materials. Continue with the SensiFAST cDNA Synthesis Kit to generate real-time ready cDNA from total RNA for complete unbiased 5’ to 3’ RNA sequence representation.

When used in combination with SensiFAST Probe and SYBR Kits, the latest advances in buffer chemistry and additives deliver ultra-sensitive real-time results in less than an hour.

Gene Expression Workflow

Gene Expression Workflow

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