- Reproducible – consistent results between technical replicates for increased confidence in results
- Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types
- Sensitive – reliable quantification of low abundance targets and scarce samples
- Fast – delivers reproducible, accurate assay results in as little as 30 minutes
- Efficient – excellent performance in multiplex assays
- Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability
The SensiFAST™ Probe Hi-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on all commonly-used real-time instruments that require a high concentration of the passive reference dye ROX. SensiFAST Probe has been formulated for use with dual-labelled probes, including TaqMan®, Scorpions® and molecular beacon probes.
A combination of the latest advances in buffer chemistry and PCR enhancers, together with an antibody-mediated hot-start DNA polymerase, ensures that the SensiFAST Probe Kit produces reliable assay results under fast thermal cycling conditions. Furthermore, SensiFAST Probe has been optimized to deliver excellent results in multiplex assays.
The SensiFAST Probe Hi-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.
- Gene expression analysis
- DNA / RNA target detection
- miRNA profiling / quantification
- Copy number variation (CNV) analysis
I performed a comparison of Bioline's SensiFAST Probe against several other qPCR master mixes. The Bioline product performed the best overall in terms of efficiency, sensitivity, lower and cost. I have also been very impressed Bioline's customer service.
Tiffany Kosch, James Cook University, Townsville, Australia
Instrument CompatibilityPlease refer to the Real-Time PCR Selection Chart to confirm the SensiFAST Probe Hi-ROX Kit is compatible with your instrument.
Certification of Analysis (COAs)
200 x 20 µL Reactions
500 x 20 µL Reactions
2000 x 20 µL Reactions
SensiFAST Probe Hi-ROX Mix (2x)
2 x 1 mL
5 x 1 mL
4 x 5 mL
- BIO-82002: 250 x 20 µL Reactions: 2 x 1 mL
- BIO-82005: 500 x 20 µL Reactions: 5 x 1 mL
- BIO-82020: 2000 x 20 µL Reactions: 4 x 5 mL
Storage & Stability
All kit components should be stored at -20°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label. Avoid exposure of the ROX™ to light.
Shipped on dry/blue ice.
Human - Rosato R.R., et al. Cancer Res. 67: 9490-500 (2007)
Rat - Remund K., et al. Expt. Lung Res. 35: 359-370 (2009)
Drosophila - Brown A.E., et al. PloS ONE 4: e4490 (2009)
Nitrogen-fixing bacteria - Bahlawane C., et al. Mol. Plant-Microbe Inter. 21: 1498-1509 (2008)
Diphtheria bacteria - Jochmann N., et al. Microbiology 155: 1459-1477 (2009)
Green algae - Wobbe L., et al. PNAS 106: 13290-13295 (2009)
Bovine virus - Park S-I., et al. J. Virol. Methods. 159: 64-6 (2009)
For cDNA/DNA templates SensiFAST SYBR® and Probe kits are used. To give you some idea of a few of the types of templates used with these kits:
Stem Cell - Bernardo A.S., et al. Stem Cells 27(2): 341 -351 (2008)
Cow - Baumert A., et al. J. Dairy Research 76(3): 356-364 (2009)
Mouse - Hoyles R.K., et al. Arthritis Rheum. 58(4): 1175-88 (2008)
Quail - De Winter P., et al. British Poultry Science 49(5): 566 – 573 (2008)
Insect - Bass C., et al. Malaria journal 6 111 (2007)
Fish - Miller M.R., et al. J. Nutr. 138(11): 2179-2185 (2008)
Crab - Wilcockson D.C. & Webster S.G. Gen. & Comp. Endo. 156: 113–125 (2008)
Nematode - Murray S.L., et al. Mol. Plant-Microbe Interactions 20(11): 1431–1438 (2007)
Plant - Hecht V., et al. Plant Physiology Preview DOI:10.1104/pp.107.096818 (2007)
Yeast - Kawauchi J., et al. Genes & Dev. 22: 1082-1092 (2008)
E.coli Bacteria - Saeed H.A., et al. Research J. Microbiology 4(4); 173-177 (2009)
Virus - Muscillo M., et al. Water, Air, & Soil Pollution 191: 1-4 (2008)