| Sign In | Quick Order CART (0)
  • EPIK™ miRNA Panel Assays

    Using advanced design concepts, a proprietary algorithm has been developed by MiRXES™ to create optimal primer combinations for the EPIK™ miRNA Panel Assays, to maximize miRNA detection sensitivity while minimizing non-specific interactions. The assays use SYBR® Green rather than a probe-based system for detection, allowing rapid amplification when required. The resulting real-time PCR assays enable detection of extremely low levels of miRNA with high specificity, allowing the discrimination between closely related miRNA sequences.
     
    All EPIK™ miRNA Panel Assays have been validated using both synthetic miRNA templates and human total RNA. Typically the assays detect as few as 100 copies of template per RT reaction with excellent assay efficiency and linearity to produce complete systems for miRNA profiling of cancer samples, biofluids and stem cells.

    • Increased sensitivity for limiting amounts of input sample
    • Improved specificity gives superior discrimination of even very closely related targets
    • Faster protocol for earlier results and increased throughput
    • Accurate quantification of low and high expressed targets in the same sample
    • Conveniently formulated to minimize set-up time

    Fact Sheet

    EPIK miRNA Panels

    Poster

    Discovering Circulating MicroRNA Biomarkers...

    Introduction to EPIK Panels

    Overview, features and benefits of the EPIK Panels

    EPIK™ miRNA Panel and Select Assay Workflow

    A summary of how to perform the assay protocol.

    EPIK™ miRNA Select Assays

    Bioline offers an extensive list of individual human miRNAs, using the proprietary algorithm developed by MiRXES™ to maximize miRNA detection sensitivity, while minimizing non-specific interactions. The assays use SYBR® Green rather than a probe-based system for detection, allowing rapid amplification when required. The resulting real-time PCR assays enable detection of extremely low levels of miRNA with high specificity, allowing the discrimination between closely related miRNA sequences.
     
    All EPIK™ miRNA Select Assays have been validated using both synthetic miRNA templates and human total RNA. Typically the assays detect as few as 100 copies of template per RT reaction with excellent assay efficiency and linearity.

    • Increased sensitivity for limiting amounts of input sample
    • Improved specificity gives superior discrimination of even very closely related targets
    • Faster protocol for earlier results and increased throughput
    • A wide range of pre-validated assays available off-the-shelf
    • Custom assays designed and validated upon request
    • Conveniently formulated to minimize set-up time

    “The experimental procedure of the EPIK™ miRNA Select Kit is very easy. Multiplex cDNA synthesis for 3 miRNA targets and 1 reference target is highly efficient and led to very stable and reproducible results in QRT-PCR.”

    Professor Dr. Peter Bugert, Institut für Transfusionsmedizin und Immunologie Mannheim, Germany